IMPORTANT NOTE: The updated version of PISTACHIO along with secondary structure determination is available through PINE webserver. We strongly recommend using PINE webserver. Otherwise you can still use this old version of PISTACHIO.       PISTACHIO Server v. 0.1 (Beta) PISTACHIO is a component in a suite of tools for high-throughput structure determination using NMR. PISTACHIO with extended laboratory management functionality will be available through the SESAME software. PISTACHIO currently achieves better than 90% correct assignments for backbone and sidechains.     A service offered by the National Magnetic Resonance Facility at Madison.  PISTACHIO offers fully automatic backbone and sidechain assignments using a probabilistic approach that achieves excellent results. NOTE:  Input file format is peak-picked resonance data in the XEasy format. A sample of xeasy files and a short instruction are available at here.   Assignment results, including secondary structure identification, are returned via email .    Depending on the size of the protein, the quality of input data, and number of current jobs, run times vary from one hour to one day.      Updated AUGUST 2005.

Instructions

SUBMIT ALL FILES IN XEASY FORMAT.