HiFi
HIFI (High Resolution Iterative Frequency Identification for NMR)
PINE accepts as input, the sequence of the protein plus peak lists (or spin systems) from a variety of NMR experiments and offers automatic backbone and sidechain assignments, detection and automated correction of potential referencing problems or inconsistent assignments and secondary structure determination.
Acceptable formats for input data are:
1. Amino acid sequences
and peak lists in Sparky format, XEASY format, or PINE
simple format.
Sample files and instructions are available
here.
2. If you have already
analyzed the spin systems, you can upload your
spin file in correct format in the
spin system field at the end of this
webpage.
3. PINE also offers assignments for proteins that have been
selectively labeled or have prior partial assignments.
- Instruction for PINE output files can be found here.
- Assignment results, including secondary structure identification are returned via email.
- Depending on the size of the protein, the quality of input data, and number of current jobs, run times vary from one hour to one day.
UPLOAD FILES
- This form supports upload of up to 15 files with corresponding experiment names.
- You can leave unneeded file names blank.
- A short instruction page is available here.
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